Identification of cadmium-regulated genes by cDNA-AFLP in the heavy metal accumulator Brassica juncea L.
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چکیده
منابع مشابه
Identification of cadmium-regulated genes by cDNA-AFLP in the heavy metal accumulator Brassica juncea L.
In this study, cDNA-amplified fragment length polymorphism (cDNA-AFLP) analysis was employed to identify genes that exhibited a modulated expression following cadmium (Cd) treatment in Brassica juncea grown in hydroponic culture. Plants were treated for 6 h, 24 h, and 6 weeks with 10 microM Cd(NO3)2 and untreated 6-week-old plants were used as controls. Cd content was measured at these four tim...
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Plant growth, pigment concentration, biochemical parameters and uptake of heavy metals were determined for Brassica juncea L. in response to cadmium and lead stress. The plant exhibited a decline in growth, chlorophyll content and carotenoids with Cd and Pb but Cd was found to be more detrimental than Pb treatment in B. juncea. The protein content was decreased by Cd (900 μM) to 95% and 44% by ...
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Aluminium (Al) toxicity is the major factor limiting crop productivity in acid soils. To investigate the molecular mechanisms of Al toxicity and Al tolerance of rice, cDNA-amplified fragment length polymorphism (cDNA-AFLP) was used for identifying Al-regulated genes in roots of an Al-tolerant tropical upland rice, Azucena, and an Al-sensitive lowland rice, IR1552. Nineteen function-known genes ...
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In plants, nitric oxide (NO) has multiple roles in defence reactions under abiotic stresses, including heavy metal load. Literature data suggest that there is a causal relationship between NO and iron metabolism but the effects of essential micronutrients/toxic heavy metals on NO production have not been investigated. In this study our aim is to demonstrate the possible role of NO in the plant ...
متن کاملAFLP markers linked to seed quality traits in Brassica juncea
Canola quality Brassica juncea has been under development at the Saskatoon Research Centre for several years. Breeding efforts have focused on the development of germplasm with improved seed quality characteristics including superior fatty acid profiles, low glucosinolates and high oil content. Future breeding efforts in this crop would be greatly facilitated through the development of molecula...
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ژورنال
عنوان ژورنال: Journal of Experimental Botany
سال: 2005
ISSN: 1460-2431,0022-0957
DOI: 10.1093/jxb/eri299